|
KMID : 0352719990230020074
|
|
Journal of Ginseng Research
1999 Volume.23 No. 2 p.74 ~ p.80
|
|
|
Origin and Development of Single- and Poly-embryos formed Directly on Excised Cotyledons of Ginseng Zygotic Embryos
|
|
양덕춘/Deok Chun Yang
Kwang Tae Choi
|
|
|
Abstract
|
|
|
|
Excised cotyledon segments of ginseng zygotic embryos cultured on MS basal medium without growth regulators produced somatic embryos near the basal excised portion at a high frequency. The frequency of somatic embryo formation on the segments declined along with advancing zygotic embryo maturity. In immature cotyledons, all the cells of the epidermis and subepidermis were smaller and more densely cytoplasmic than those in mature cotyledons, and from which multiple cells participated in embryogenic division to form somatic embryos with multiple cotyledons and fasciated radicles (poly-embryos). But in germinating cotyledons, only the epidermal cells were densely cytoplasmic and singularly competent to develop into somatic embryos resulting in single-embryos with closed radicles. This result means that the origin and development of somatic embryos is determined according to whether the cells participating in embryonic division are in a single state or a massive state relative to cotyledon maturity.
|
|
|
KEYWORD
|
|
|
Origin of somatic embryo, Cotyledon segment, Maturity of explant, Panax ginseng,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|